Stunner AF
The only plate-based dynamic light scattering, UV/Vis and fluorescence tool built for LNPs
Conquer LNP characterization with DLS, UV/Vis & fluorescence
Stunner AF adds fluorescence detection to plate-based, high-throughput rotating angle dynamic light scattering (DLS) and UV/Vis absorbance measurements from 2 µL on up to 96 samples. Combine all three technologies to measure LNP size, encapsulation efficiency (EE%), and particle concentration data in one experiment. Pivot and pair up UV/Vis and DLS to quantify and size proteins, ADCs, peptides - just like Stunner.
• Size & polydispersity: See whether your LNP's diameter is what you expect and how uniform it is - critical for screening, formulation, and QC.
• Encapsulation efficiency: Combine free RNA quantification from fluorescence and total RNA quantification from UV/Vis to make EE% characterization extra easy, detergent-free, and ready for high-throughput.
• Particle concentration: Sum up the total number of LNPs present in your samples to compare LNP production from one condition or batch to any other.
• Aggregate detection: RADLS is the most sensitive way to pick up on unwanted particles and samples that have gone south, so you can fix issues fast.
• Conjugate characterization: See how much protein is along for the ride by quantifying protein concentration at the same time you're characterizing your LNP.
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Check out LNP characterization with Stunner
Take a peek at LNP encapsulation efficiency with Stunner
Revolutionize EE%
Want fluorescence? Stunner AF (Add Fluorescence) delivers UV/Vis with sizing data and takes it up a notch with fluorescence for dye-based quantification of free RNA. Load up standards, LNPs, and LNPs mixed with RiboGreen for Total RNA and Free RNA quant. Build up your own assays or grab EE% plus sizing to rip through LNP characterization with our optimized applications.
See through the fog
Cloudy solutions of LNPs and other nanoparticles hang up other techniques but Stunner's short pathlengths teamed up with UV/Vis get you the answers you need. Cut through all that turbidity with Unmix and check out just the absorbance signal from your payload.
LNP sizing & quant in one shot
Stunner sizes up and counts your LNPs by combining DLS and SLS data from a bunch of different angles. Compare different formulations and mixing parameters to see which one gets you the perfect LNP size - and from the same run, Stunner clocks which contain the most particles too.
Know what's up
Stunner can catch even the tiniest bit of aggregation. Check out your LNP size by intensity distribution first and stay on the lookout for aggregates. If you see aggregation rearing its ugly head, make the switch to mass distribution to see how bad it might be. Beef up your sizing stats and never get fooled by rogue aggregates again.
Screen at light speed
Stunner is the ultimate tool for LNP screening and rapid characterization, delivering all the need-to-know info on up to 44 LNP samples on a single plate in about 2 hours. Combine UV/Vis, fluorescence, DLS and SLS from multiple angles to get size, EE%, total payload concentration, and particle concentration. Stunner always tells the whole lipid nanoparticle story.
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Specification
| Stunner instrument specifications | |
|---|---|
| Dimensions | Stunner: 37 cm W x 54 cm D x 33 cm H; 30.4 kg Stunner AF: 37 cm W x 58 cm D x 33 cm H; 30 kg |
| Electrical | Universal input voltage 100-240 V AC, 50-60 Hz |
| Computer | Separate computer with Windows 11 included |
| Connection | USB, TCP/IP (Service) |
| Approval | CE, FCC, CSA |
| Regulatory compliance | Optional 21CFRp11 software packagee; USP and Ph. Eur. Performance verification standards |
| UV/Vis | ||
|---|---|---|
| Light source | Xenon flash lamp | |
| Detectors | UV/Vis polychromatic spectrophotometer | |
| Wavelength range | 230-750 nm | |
| Wavelength accuracy | ≤400 nm: ±1 nm; ≥400 nm: ±2 nm | |
| Spectral resolution | Better than 2 nm (toluene in hexane) | |
| Absorbance precision (1 cm quartz cuvette) | <1 OD: ±0.005 OD st dev | 1-2 OD: ±0.5% CV |
| Absorbance accuracy (1 cm quartz cuvette) | <1 OD: ±0.01 OD | 1-2 OD: ±1% |
| Fluorescence (Stunner AF only) | |
|---|---|
| Channels | Blue/Green (Ex. 475 nm LED/Em. 515–565 nm) Red/Far Red (Ex. 624 nm LED/Em. 672–712 nm) |
| DLS and rotating angle DLS | |
|---|---|
| Light source | 2 x 660 nm laser diodes |
| Detection | Avalanche photodiode module |
| Number of angles | 1 (DLS), 5 – 30 (RADLS) |
| Angular range | 30–42° & 110–162° |
| Size accuracy | ±2% |
| Minimum sample concentration | 0.1 mg/mL lysozyme |
| Hydrodynamic diameter range | 0.3–1000 nm |
| Particle concentration range | 109 – 5x1013 particles/mL (dependent on particle size, determined on 80 nm beads) |
| Stunner plate specifications | ||
|---|---|---|
| Samples per plate | 96 (12 x 8 microplate format) | |
| Sample retention time | Up to 2 hours | |
| Recommended sample volume | 2 µL | |
| Pathlength(s) | 0.1 mm & 0.7 mm path | |
| Measurement time for full plate | ~10 minutes for UV/Vis only ~1 hour for UV/Vis and DLS (5 x 4s x 1 angle) ~2 h 15 minutes for UV/Vis and RADLS (5 x 1s x 7 angles) |
|
| Measurement range: OD 10 mm ng/µL dsDNA mg/mL ave protein mix | 0.03–275 OD 10 mm 1.5–13750 ng/µL 0.03–275 mg/mL |
|
| Absorbance precision (10 mm pathlength) | <1 OD: ±0.01 OD st dev | 1–200 OD: ±1% CV |
| Absorbance accuracy (10 mm pathlength) | <1 OD: ±0.02 OD | 1–200 OD: ±2% |
