Sunshine
The all-around LNP process developer
Scale it up
Once you've found a rock-solid formulation, Sunshine helps you tune in your LNP's size and design the perfect process to scale them up. Scout ideal flow rates, explore different mixing options, then dial in the right dilution factor to keep them stable. When you've got everything just right, Sunshine can shift into continuous mode, making tens of liters in a day. Get process development and scale-up on one platform - who would have figured? Get brochure.
- 10 experiments in 15 minutes
- Fractional & total flow rates
- 1 mL to continuous flow
- 0.1 - 30 mL/min flow rates
- Reusable microfluidics
- Protocol transfer from Sunscreen
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Watch Sunshine in action.
Find your flow
Fill one syringe with payloads, the other with lipids and you're ready to mix them up with a Sunny. For a simple, well-known T-mixing approach grab a Sunny T, or to increase fluid contact area for particle formation with a cross-junction, use a Sunny X. To use Sunshine's full range of flow rates, ratios and integrated in-line dilution, choose a Sunny Trident. Sunnies can be subbed in and out of Sunshine, used over and over, and when you're ready to turn up the volume, they can run longer than you can.
Fine-tune it fast
Tell Sunshine's Sunny Suite Software what's in your syringes, the lipid to payload ratios, the flow rates you want to mix at, how much diluent you want to add and you're ready to go. The software checks to make sure your experiments will work, so you don't need to worry about screwing things up. Create 10 different samples in 15 minutes or let Sunshine run for hours while it makes liters of LNPs.
Dial it in
From their ability to get inside of cells, to releasing their payload, size really does matter when it comes to LNPs. Sunshine fully automates the fractional flow rate, total flow rate and dilution experiments that helps you size them down and keep them stable, with spot on repeatability. Tinker with up to 10 parameters per run and let Sunshine take it from there.
Bulk it up
Sunshine is ready when you are to start making large volumes of LNPs. Whether you're making milliliters or liters, your sizes and PDIs will stay consistent - no matter how much you decide to make.
The Sunny Suite
The Sunny Suite has a killer solution for every step of your LNP development. Sunscreen automates high-throughput screening of up to 96 formulations at a time to help you quickly discover your winning LNP formulations. Sunshine then takes your best formulations and hones in on the perfect mixing process conditions, with both automated experiments and continuous flow modes. When you're ready for clinical trials, Sunbather dials up your particles in a fully GMP-compliant environment.
Trust the process
Using the same pumps for fluid delivery, and same range of Sunnies for mixing up your formulations, the Sunny Suite keeps every transition from formulation screening to process development to GMP production smooth sailing. Whether you're screening through hundreds of 200 μL formulations or producing liters of particles for clinical trials, you can trust the Sunny Suite to deliver the same consistent results at any scale.
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App Notes
Specification
| Application | Sunshine | Sunshine Lite | Sunshine Single |
|---|---|---|---|
| Throughput | 10 samples in 15 minutes | 10 samples in 15 minutes | 1 sample in 5 minutes |
| Total flow rate range (Sunny dependent) | 0.1–30 mL/min | 0.1–20 mL/min | 0.1–20 mL/min |
| Typical sample volume (3:1 FRR) | ≥1 mL | ≥1 mL | 0.4–6.3 mL |
| Minimum input volume (including dead volume) | ≥170 µL | ≥170 µL | ≥170 µL |
| Continuous mode | Yes | Yes | No |
| In-line dilution | Yes | No | No |
| Flow rate ratio range (aqueous to organic) | 1:1 to 10:1 | ||
| Typical particle size | 40–200 nm* | ||
| PDI | <0.2* | ||
| Encapsulation efficiency | >90%* | ||
| Instrument | |||
|---|---|---|---|
| Physical | |||
| Weight and dimensions (cm) | 31 kg, 53 H x 60 W x 40 D | 23 kg, 53 H x 40 W x 40 D | 20 kg, 35 H x 40 W x 40 D |
| Electrical | |||
| Voltage input | 100 V–240 V AC, 50–60 Hz | ||
| Communications | USB | ||
| Other information | |||
| Sample loop size | 1, 5 and 10 m | 1, 5 and 10 mL | 5 mL |
| Wetted materials | PTFE, PCTFE, FEP, ETFE, PEEK, Polypropylene, FFKM, Hastelloy C276, Stainless Steel T316, Glass, Tygon (waste tubes), PET (waste bottle) |
||
| Compute | Separate computer with Win 11, monitor, keyboard and mouse (not included in Dimensions) |
||
| Consumable | |
|---|---|
| Sunnies | Glass microfluidic mixing devices of various geometries and channel sizes |
| Available types | Sunny 490 Trident T, Sunny 100 X, Sunny 100 T, Sunny 190 X, Sunny 190 T, Sunny 275 X, Sunny 275 T, Sunny 150 3D, Sunny 50 Micromixer |
